Journal: Vaccine

Article Title: Oral immunization with LacVax ® OmpA induces protective immune response against Shigella flexneri 2a ATCC 12022 in a murine model

doi: 10.1016/j.vaccine.2019.04.053

Figure Lengend Snippet: Bacterial strains and plasmids used in the study.

Article Snippet: Shigella flexneri 2a (ATCC 12022) , Pathogenic strain responsible for shigellosis , Lab Source NCBI Accession No.: KX826786.

Techniques: Plasmid Preparation, Sequencing

Journal: Vaccine

Article Title: Oral immunization with LacVax ® OmpA induces protective immune response against Shigella flexneri 2a ATCC 12022 in a murine model

doi: 10.1016/j.vaccine.2019.04.053

Figure Lengend Snippet: Pathophysiological parameters of immunized animals following intraperitoneal (i.p.) administration of S. flexneri 2a. (A) Photographs of anal region of representative animal from each group following S. flexneri 2a infection. The mucus secretion in the OS and OW group of mice indicate bacterial dysenteriae. (B) Photograph of bacterial conjunctivitis in OS group of mice, a hallmark of systemic infection of S. flexneri 2a in BALB/c mice. (C) Representative photographs of the colon of immunize animals before and after S. flexneri 2a infection. (D) Comparison of the colon lengths (in mm) of all immunized animals before and 24 h after S. flexneri 2a infection ( * P < 0.05).

Article Snippet: Shigella flexneri 2a (ATCC 12022) , Pathogenic strain responsible for shigellosis , Lab Source NCBI Accession No.: KX826786.

Techniques: Infection, Comparison

Journal: Vaccine

Article Title: Oral immunization with LacVax ® OmpA induces protective immune response against Shigella flexneri 2a ATCC 12022 in a murine model

doi: 10.1016/j.vaccine.2019.04.053

Figure Lengend Snippet: Histopathological changes in the small and large intestine of immunized animals following intraperitoneal (i.p.) administration of S. flexneri 2a. (A) Blind scoring of H & E stained samples of small and large intestine. Scoring is based on overall tissue structure and integrity. The score ranges from 0 to 4 indicating the severity of disease characteristics. (B) The tissue sections of all the animals were further assessed for particular parameters; (I) Cell death in epithelium, (II) Cell death in lamina propria, (III) Epithelium exfoliation, (IV) Cell death in crypt, (V) Cell death in muscle layer, (VI) Whole tissue integrity and blindly scored from 0 to 4 where 0 indicates minimal change and 4 indicated maximum disruption.

Article Snippet: Shigella flexneri 2a (ATCC 12022) , Pathogenic strain responsible for shigellosis , Lab Source NCBI Accession No.: KX826786.

Techniques: Staining, Disruption

Journal: BMC Microbiology

Article Title: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

doi: 10.1186/1471-2180-10-72

Figure Lengend Snippet: Non- Francisella organisms from the strain collection of the Bundeswehr Institute of Microbiology tested to assess the specificity of the FISH assay

Article Snippet: Shigella flexneri , DSMZ 4782 , + , - , -.

Techniques:

Journal: BMC Microbiology

Article Title: Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics

doi: 10.1186/s12866-015-0577-3

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Vibrio fluvialis , ATCC 33809 , 14 , Shigella flexneri 2a strain 2457 T , ATCC 700930 , 36.

Techniques: Bacteria